Technique Comparison
In a two-dimensional scatter plot, the data of thermodynamic parameters obtained by different instrumental techniques are compared.


Tooltips

Positioning the mouse cursor on the page element, the user can obtain a short description in the tooltip.


Selecting thermodynamic parameter

Users can select the thermodynamic parameter to show in the graph window.
Default is the observed dissociation constant(Kd,obs).


Selecting methods on axes

The four instrumental methods can be set on the X and Y axes.
Comparing two different methods leads to exposing the limit set of data points where experiments of protein-ligand pairs intersect.
Use the same selection on both axes to show the entire data set for a single instrumental method.


Selecting proteins

For selecting exposable protein groups, use Clear all and Set all buttons or checkboxes for individuals.


Set colors of data points

Colors of data points can be set according to protein group or protein-ligand binding strength.


Filters

Case insensitive search by compound name fragment. After writing the string in the edit line, mouse-click on OK button or press the Enter key.


Loading data

Source data control elements. The tool uses `all_method_kd_obs_stats_log10` and `all_method_kd_int_stats_log10` database tables (views).
a Links for quick download from the server of the current data in various file formats.
b Links for saving the data of the current data view in various file formats.
c Button enables reloading of both database tables (views).
d Shows the number of loaded database table rows.


Data point info

Information about selected data point in the graph area appears in the left panel card.
Alongside the text, one can use the links:
a link refers to a single record in the statistics table.
b and c refer to the compound record in the `compound` table and Compound browser tool, respectively.
d and e refer to the experiment records in the corresponding methods tables.


Current graph data points info

This left panel card shows short statistics for all visible data points.
Statistics dynamically change while different settings and filters are applied.


Graph card controls

a The "Fixed scale" check box switches between a fixed (maximal, default) axis scale range and a dynamic one.
b The "Fixed size" check box defines the size of a graph window. Not fixed graph window adopts in size to browser window size.
c "Show errors" check box switches on additional data point information. Thin black lines lie between minimal and maximal values. Thick green bars show the standard deviation.
d "Reset chart" button returns the zoomed graph window to its start appearance.
e "Save SVG" button downloads or opens the current graph window view (not zoomed) depending on your browser settings.


Graph card functionality

Zoom
Zoom a graph area using a mouse wheel. Pan by mouse click and move.

Tooltips
Positioning the mouse cursor on the data point, the user can obtain names of protein group and ligand used, temperature, X and Y values.

a A mouse click selects a single data point. Explicit information appears on the Data point info card on the left side.
b When the Show errors check box is checked, thin black lines show a range of the values.
c Thick green bars show the standard deviation.


API

Example:
Technique Comparison base URL: https://tools.plbd.org/tc.html
Available parameters:
compounds - list of the compound ID's in JavaScript array and Python set style [id1, id2, ...].


URL-defined compounds highlighting

Use the From URL combo box to highlight data points with compounds passed through the compounds URL parameter.
Uncheck the Only check box to show all data points.